Carbohydrates are divided into digestible carbohydrates, such as sucrose or oligofructose, and indigestible carbohydrates, such as dietary fiber or ß- glucans whose positive effects, for example in the healing of wounds and in the area of cancer therapy, have been studied.
High performance anion exchange chromatography (HPAEC) with pulsed amperometric detection (PAD) appears to be a very selective and sensitive method for the analysis of digestible carbohydrates. The separation of the carbohydrates is achieved in an alkaline environment on an ion-exchange column; the separated carbohydrates are then detected electrochemically. The measurement range lies between 1 and 20 mg/l.
Using HPAEC-PAD in combination with different enzymatic hydrolyses, a broad range of carbohydrates can be examined. A selection of various factors is listed below:
- spectrum of free mono-, di- and trisaccharides
- net carbohydrates in accordance with the Journal of AOAC International, Vol. 88, No. 3, 2005
- oligofructoses in accordance with the Journal of AOAC International, Vol. 83, No. 2, 2000
- trans-galacto-oligosaccharides (TGOS) in accordance with AOAC Official Method 2001.02
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